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藥靶細(xì)胞株 > kinase激酶細(xì)胞株 > CBP73276c-Kit D816Y/BaF3

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CBP73276 | |
I. Introduction | |
Cell Line Name: | c-Kit D816Y/BaF3 |
Host Cell: | Ba/F3 |
Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
II.Background | |
KIT D816Y lies within the tyrosine kinase domain 2 of the Kit protein (PMID: 21640708). D816Y results in constitutive phosphorylation of Kit, activation of Akt and Mapk pathways in cultured cells (PMID: 21640708, PMID: 16397263), and confers resistance to imatinib in culture (PMID: 16397263). | |
III. Representative Data | |
1. WB of c-Kit D816Y/BaF3 | |
2.Sanger of c-Kit D816Y/BaF3 | |
3. Anti-proliferation assay Figure 3. CTG Proliferation Assay of BaF3 c-KIT D816Y (C1). |